In one line
What Is PCR / Molecular?
What is PCR / Molecular testing?
Polymerase chain reaction (PCR) amplifies a DNA sequence specific to the target organism so that even a few cells in an enriched sample can be detected with high specificity. Target DNA is amplified using specific primers, enabling highly sensitive molecular detection of the organism.
Mechanism
How the Method Works
Target DNA is amplified using specific primers, enabling highly sensitive molecular detection of the organism.
SampleWeigh the food or take the environmental swab.
EnrichmentGrow the target to detectable numbers.
ExtractionRelease and purify the DNA.
AmplificationCycle with specific primers and probes.
DetectionMonitor the fluorescence signal.
ResultReport presumptive positive or negative.
Fit
When Should You Use PCR / Molecular?
Strong fit
Presumptive results within a shift after enrichment
High specificity for named pathogens
High-risk products and release testing
Consider another method when…
Microbial counts → ready-to-use media
Environmental hygiene screening → rapid enzymatic tests
Decision support
PCR / Molecular vs Other Methods
| Requirement | Rapid Enzymatic | Real-time PCR | Ready-to-Use Media | Traditional Culture |
|---|---|---|---|---|
| Speed | ★★★★★ | ★★★★ | ★★★ | ★ |
| Throughput | High | High | High | Medium |
| Result | Presence / absence | Detection | Counts | Counts |
| Specificity | Good | Very High | Good | High |
| Equipment | Low | High | Low | Medium |
| Best suited for | Environmental screening | High-risk pathogens | Routine enumeration | Reference confirmation |
Ratings are indicative - validate against your specific analyte and matrix with the Fobeventia technical team.
Unsure which column your process belongs in?
Tell us the analyte, matrix and the decision the result has to support, and a specialist will place it for you.
FAQ
Frequently Asked Questions
What is PCR / Molecular?
Polymerase chain reaction (PCR) amplifies a DNA sequence specific to the target organism so that even a few cells in an enriched sample can be detected with high specificity.
What equipment do I need?
Real-time PCR instrument, Micropipettes, DNA extraction kit and Enrichment media; optionally Automation and Laboratory consumables.
Is PCR / Molecular quantitative?
Detection (presence/absence) after enrichment; quantification is not the aim of routine food-pathogen PCR.
When should I choose another method?
- Microbial counts → ready-to-use media
- Environmental hygiene screening → rapid enzymatic tests