What Is LC-MS/MS?
How the Method Works
After cleanup, analytes are separated by LC and fragmented in the mass spectrometer; characteristic ion transitions confirm identity and quantify concentration.
When Should You Use LC-MS/MS?
LC-MS/MS vs Other Methods
| Requirement | Rapid Test | ELISA | HPLC | LC-MS/MS |
|---|---|---|---|---|
| Speed | ★★★★★ | ★★★★ | ★★ | ★★ |
| Throughput | High | High | Medium | Medium |
| Quantitative | Limited | Yes | Yes | Yes |
| Specificity | - | Good | High | Very High |
| Equipment | Low | Medium | High | Very High |
| Best suited for | Line-side screening | Routine QC | Quantitative analysis | Confirmatory analysis |
Ratings are indicative - validate against your specific analyte and matrix with the Fobeventia technical team.
Unsure which column your process belongs in?
Tell us the analyte, matrix and the decision the result has to support, and a specialist will place it for you.
Frequently Asked Questions
What is LC-MS/MS?
Liquid chromatography–tandem mass spectrometry separates analytes chromatographically and identifies them by mass, giving the highest specificity and multi-analyte capability.
What equipment do I need?
LC-MS/MS system, Immunoaffinity or SPE cleanup, Certified reference materials and Solvents and consumables; optionally Automation and Isotope-labelled internal standards.
Is LC-MS/MS quantitative?
Yes - quantitative and confirmatory.
When should I choose another method?
- Routine screening → rapid tests or ELISA