What Is HPLC?
How the Method Works
The extract is cleaned up - usually on an immunoaffinity column - injected onto the column, separated under pressure and quantified against calibration standards.
When Should You Use HPLC?
HPLC vs Other Methods
| Requirement | Rapid Test | ELISA | HPLC | LC-MS/MS |
|---|---|---|---|---|
| Speed | ★★★★★ | ★★★★ | ★★ | ★★ |
| Throughput | High | High | Medium | Medium |
| Quantitative | Limited | Yes | Yes | Yes |
| Specificity | - | Good | High | Very High |
| Equipment | Low | Medium | High | Very High |
| Best suited for | Line-side screening | Routine QC | Quantitative analysis | Confirmatory analysis |
Ratings are indicative - validate against your specific analyte and matrix with the Fobeventia technical team.
Unsure which column your process belongs in?
Tell us the analyte, matrix and the decision the result has to support, and a specialist will place it for you.
Learn More About HPLC
Frequently Asked Questions
What is HPLC?
High-performance liquid chromatography separates the analyte from the sample extract on a column and measures it with a UV or fluorescence detector for quantitative, confirmatory results.
What equipment do I need?
HPLC system with FLD/UV detector, Immunoaffinity columns, Reference standards and Solvents and consumables; optionally Post-column derivatisation and Autosampler.
Is HPLC quantitative?
Yes - fully quantitative against calibration standards.
When should I choose another method?
- Routine screening → rapid tests or ELISA
- Multi-analyte panels → LC-MS/MS